Abstract: OBJECTIVE: To investigate the expressions of IL-28 and mac-3 in liver of chronic alcoholism mice and withdraw from alcohol. METHODS：The dose of 40% alcohol [8 mg/ (g·d)] was administered via gastrolavage once daily for 12 weeks in Kunming mice. The control mice were given the same volume of saline. The experimental mice were sacrified at the end of 4th，8th，12th week and after withdrawal from alcohol 24 hours. Pathologic changes of liver were examined under light microscope after HE staining，expressions of IL-28 and mac-3 were examined by immunohistochemitry. RESULTS：The tissues of treated mice showed various changes of chronic alcoholic hepatitis at the end of 4，8，12 week，such as fatty degerneration，inflammatory and fibrosis，more severe with longer alcohol administration. The expressions of IL-28 and mac-3 were decreased in alcoholic mice but increased in mice withdrawal from alcohol. CONCLUSION：Alcohol could injure Kupffer cells or inhibit their functions. Withdrawal of alcohol may introduce the rebound of IL-28 and mac-3 expressions or temporary inflammation of liver.

Key words: alcoholism, withdrawal from alcohol, IL-28, mac-3